In this work we aimed to examine the influence of the main abiotic factors (temperature, rainfall, relative humidity, atmospheric
pressure) on the synthesis of active compounds (phenolics, flavonoids), with antioxidant and therapeutic effect of two herbs -
Capsella bursa-pastoris (shepherd's purse) and Marrubium vulgare (horehound) collected from two different areas of Romania:
Dobrogea (Constanta) and Muntenia (Bucharest). The studies have shown that biogenesis and accumulation of secondary
metabolites that gives the therapeutic properties of medicinal plants is influenced by biotic and abiotic factors. In this work we
aimed to determine the profile of concentrations of biologically active compounds from the studied herbs depending on the harvest
time period and variability of the environmental factors. The medicinal herbs had harvested in autumn (September, 2013) and in
spring (May – June, 2014); the alcoholic and aqueous extracts were analyzed in terms of composition: in main biologically active
compounds (phenolics, flavonoids) and antioxidant activity as DPPH and ABTS, and spectral data analysis by FTIR. Experimental
data obtained showed a significantly higher phenolics and flavonoids content and also a higher antioxidant activity in herbs
harvested from the Dobrogea (Constanta) area, for the herbs studied.